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Sigma
Fluorescein isothiocyanate isomer I
F7250 -
suitable for protein labeling, ≥90% (HPLC), powder
DOWNLOAD MSDS (PDF)Synonym: FITC, Fluorescein 5-isothiocyanate
CAS Number: 3326-32-7
Empirical Formula (Hill Notation): C21H11NO5S
Molecular Weight: 389.38
Beilstein Registry Number: 1407295
EC Number: 222-042-0
MDL number: MFCD00005063
PubChem Substance ID: 24894933
Properties
| Related Categories | Antibody Labeling, Antibody Modification, Core Bioreagents, E-F, Fluorescein and Derivatives, |
| assay | ≥90% (HPLC) |
| form | powder |
| fluorescence | λex 492 nm; λem 518 nm (green) |
| mp | >360 °C(lit.) |
| suitability | suitable for protein labeling |
| storage temp. | 2-8°C |
Description
Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm.
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)
Price and Availability
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Documents
Certificate of Analysis
Certificate of Origin
| F7250 - Product Information Sheet (82 KB) |
| Plant Biotech Guide (186 KB ) |
| FT-IR Raman |  |
| FT-NMR |  |
| Structure Search |  |
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Safety Information
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317-H334 |
| Precautionary statements | P261-P280-P342 + P311 |
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| WGK Germany | 3 |
Technical information & documentation associated with this product is available in the Safety & Documentation tab.
Articles
Sensor Dyes
In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Microscopy, Neurotransmission, Sol-gel
References
, E. Harlow and D. Lane, ed. Antibodies: A Laboratory Manual Cold Spring Harbor, NY , (1988), 353-355
Miller, L., Protein modification of actin at lysine-61. Eur. J. Biochem. 174, 23, (1988)
Der-Balian, G.P., FITC labelling of Fab'. Anal. Biochem. 173, 59, (1988)
Wilderspin, A.F., Green, N.M., Modification of thiol groups. Anal. Biochem. 132, 449, (1983)
Muramoto, K., Microsequencing of proteins and peptides (HPLC). Anal. Biochem. 141, 446, (1984)
Merck 14,4089
Aldrich MSDS 1, 948:C / Corp MSDS 1 (1), 1688:D / FT-IR 1 (2), 469:C / FT-IR 2 (3), 4153:C / FT-NMR 1 (2), 1560:B / IR-Spectra (2), 995:C / IR-Spectra (3), 1142:D / NMR-Reference 2 (2), 440:C / RegBook 1 (2), 2157:I / Sigma FT-IR 1 (2), 148:B / Stains and Dyes Ref, 377 / Structure Index 1, 445:C:5
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